公司注销药品生产批文转让ﾗ8￶8期报告

来源：蜘蛛抓取(WebSpider) 时间：2016-05-09 13:46 标签： 保健食品批文转让
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苏常柴的“健康之路”
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, 1 February 2013, Pages 65–69
Endo-β-N-acetylglucosaminidase H de-N-glycosylation in a domestic microwave oven: Application to biomarker discovery, , , , , , , , , , , , , , a Institute of Laboratory Medicine, Clinical Chemistry, and Pathobiochemistry, Charit& Medical University, 13353 Berlin, Germanyb Freie Universit&t Berlin, Fachbereich Biologie, Chemie, Biochemie, Pharmazie, 14195 Berlin, Germanyc Department of Gynecology, Charit& Medical University, 13353 Berlin, Germanyd Department of General, Vascular, and Thoracic Surgery, Charit& Medical University, 12200 Berlin, GermanySample preparation is the rate-limiting step in glycan analysis workflows. Among all of the steps, enzymatic digestions, which are usually performed overnight, are the most time-consuming. In the current study, we report an economical and fast preparation of N-glycans from serum, including microwave-assisted enzymatic digestion in the absence of denaturing chemicals and solvents during the release. To this end, we used a household microwave oven to accelerate both pronase and endo-β-N-acetylglucosaminidase H (Endo H) digestions. Purification was then performed using self-made SP20SS and carbon tips. We were able to prepare samples in 55 min instead of 21 h. Finally, the method was applied in the context of oncological biomarker discovery exemplarily to ovarian and colon cancer. We observed a significant downregulation of sialylated hybrid structures in ovarian cancer samples using capillary electrophoresis&laser-induced fluorescence (CE&LIF). Furthermore, sepsis, a systemic inflammatory response syndrome, was also included in the study to understand whether the changes observed in ovarian cancer patients were due to the cancer itself or to the inflammation that usually accompanies its development. Because sialylated hybrid structures were upregulated in sepsis samples, the downregulation of these structures in ovarian cancer is specific to the cancer itself and, therefore, could be used as a biomarker.KeywordsEnzymatic digestion; Microwave; Endo H; N-Glycan; Cancer
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